Elongation properties of vaccinia virus RNA polymerase: Pausing, slippage, 3' end addition, and termination site choice Journal Article
| Authors: | Deng, L.; Shuman, S. |
| Article Title: | Elongation properties of vaccinia virus RNA polymerase: Pausing, slippage, 3' end addition, and termination site choice |
| Abstract: | We have analyzed the elongation properties of vaccinia virus RNA polymerase during a single round of transcription in vitro. RNA-labeled ternary complexes were halted at a unique template position located upstream of a T-run (TTTTTTTTT) in the nontemplate strand; this element encodes an RNA signal for factor-dependent transcription termination at distal sites on the template. The halted ternary complexes were purified and allowed to resume elongation under a variety of conditions. We found that the T-run constituted a strong elongation block, even at high nucleotide concentrations. The principal sites of pausing were at a C position situated two nucleotides upstream of the first T in the T-run and at the first three to four T positions within the T-run. There was relatively little pausing at the five downstream Ts. Intrinsic pausing was exacerbated at suboptimal nucleotide concentrations. Ternary complexes arrested by the T-run at 10 μM NTPs rapidly traversed the T-run when the NTP pool was increased to 1 mM. Limiting GTP (1 μM) resulted in polymerase stuttering at the 3' margin of the T-run, immediately prior to a templated G position; this generated a ladder of slippage synthesis products. We found that vaccinia ternary complexes remained intact after elongating to the very end of a linear DNA template and that such complexes catalyzed the addition of extra nucleotides to the 3' end of the RNA chain. The 3' end addition required much higher concentrations of NTPs than did templated chain elongation. Finally, we report that factor- dependent transcription termination by vaccinia RNA polymerase downstream of the T-run was affected by nucleotide concentration. Limiting UTP caused the polymerase to terminate at sites closer to the UUUUUNU termination signal. This is consistent with the kinetic coupling model for factor-dependent termination. |
| Keywords: | signal transduction; carboxy terminal sequence; genetic transcription; transcription, genetic; enzyme activation; enzyme activity; transcription factors; methyltransferases; molecular sequence data; virus rna; rna, messenger; vaccinia virus; base sequence; dna, viral; dna flanking region; catalysis; dna primers; multienzyme complexes; phosphoric monoester hydrolases; protein tertiary structure; repetitive sequences, nucleic acid; rna, viral; electrophoresis, polyacrylamide gel; dna-directed rna polymerases; rna polymerase; vaccinia; nucleotides; dna template; nucleotidyltransferases; templates, genetic; priority journal; article |
| Journal Title: | Biochemistry |
| Volume: | 36 |
| Issue: | 50 |
| ISSN: | 0006-2960 |
| Publisher: | American Chemical Society |
| Date Published: | 1997-12-16 |
| Start Page: | 15892 |
| End Page: | 15899 |
| Language: | English |
| DOI: | 10.1021/bi972037a |
| PUBMED: | 9398322 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
