Establishment of an experimental intrapulmonary tumor nodule model Journal Article
| Authors: | Wang, H. Y.; Ross, H. M.; Ng, B.; Burt, M. E. |
| Article Title: | Establishment of an experimental intrapulmonary tumor nodule model |
| Abstract: | Background. A pulmonary tumor model is necessary to study the biology and therapy of lung cancer. Methods to establish a solitary intrapulmonary nodule are not well defined. Two methods for solitary intrapulmonary tumor nodule development in the Fischer rat are described. Methods. Methylcholanthrene-induced sarcoma cell suspensions were introduced into lung parenchyma of Fischer rats via limited thoracotomy and lung puncture, or instilled into a distal airway after tracheal puncture and catheterization. Intrapulmonary tumor location, implantation mortality, procedure length, and animal survival were recorded. Results. Single pulmonary nodules developed at the implanted position in 100% (n = 320) and 95% (62/65) of animals after direct injection into the pulmonary parenchyma or via tracheal puncture and instillation. Operative mortality was 2% and 5% via lung or tracheal implantation, respectively. Less than 5 minutes was required for each implantation. Mean survival time was 4 ± 2 and 26 ± 6 days after lung or tracheal implantation in animals allowed to survive until tumor-induced death. Conclusions. These easily performed, reproducible methods of establishing solitary intrapulmonary tumors are useful tools for lung cancer research. |
| Keywords: | survival; controlled study; nonhuman; mouse; animals; lung neoplasms; animal experiment; animal model; lung cancer; tumor cells, cultured; cancer research; lung tumor; rats; neoplasm transplantation; disease models, animal; rats, inbred f344; sarcoma, experimental; coin lesion, pulmonary; priority journal; article |
| Journal Title: | Annals of Thoracic Surgery |
| Volume: | 64 |
| Issue: | 1 |
| ISSN: | 0003-4975 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1997-07-01 |
| Start Page: | 216 |
| End Page: | 219 |
| Language: | English |
| DOI: | 10.1016/s0003-4975(97)00343-3 |
| PUBMED: | 9236364 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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