Microsatellite instability in breast cancer Journal Article
| Authors: | Rush, E. B.; Calvano, J. E.; Van Zee, K. J.; Zelenetz, A. D.; Borgen, P. I. |
| Article Title: | Microsatellite instability in breast cancer |
| Abstract: | Background: Microsatellites are short repetitive nucleotide sequences that, through mutation, can undergo either expansion or contraction. This novel mutational mechanism known as microsatellite instability may play a role in carcinogenesis. We investigated the incidence of microsatellite instability in a series of primary breast carcinoma surgical specimens. Methods: Using polymerase chain reaction techniques followed by polyacrylamide/urea gel electrophoresis, we analyzed 46 pairs of normal and primary breast tumor samples at seven different microsatellite loci, five of which were located on chromosome 17. Results: Thirteen of our 46 tumors (28.2%) demonstrated microsatellite instability. Five tumors (10.8%) were unstable at two or more loci, and of those, four (8.7%) were unstable at different loci on different chromosomes. An additional five tumors demonstrated loss of heterozygosity alone when compared with their normal counterparts. Conclusions: These findings indicate that microsatellite instability is present in primary breast cancer populations and, although the mechanism of action has yet to be elucidated, may play a role in breast carcinogenesis. Published by Lippincott-Raven Publishers © 1997 The Society of Surgical Oncology, Inc. |
| Keywords: | genetics; mutation; polymerase chain reaction; pathology; breast neoplasms; heterozygote; microsatellite dna; dna; breast tumor; breast carcinoma; dna, neoplasm; chromosome 17; polyacrylamide gel electrophoresis; electrophoresis, polyacrylamide gel; microsatellite repeats; chromosomes, human, pair 17; microsatellite; instability; humans; human; female; article |
| Journal Title: | Annals of Surgical Oncology |
| Volume: | 4 |
| Issue: | 4 |
| ISSN: | 1068-9265 |
| Publisher: | Springer |
| Date Published: | 1997-06-01 |
| Start Page: | 310 |
| End Page: | 315 |
| Language: | English |
| PUBMED: | 9181230 |
| PROVIDER: | scopus |
| DOI: | 10.1007/BF02303580 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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