Crystallization of the RNA guanylyltransferase of Chlorella virus PBCV-1 Journal Article
| Authors: | Doherty, A. J.; Håkansson, K.; Ho, C. K.; Shuman, S.; Wigley, D. B. |
| Article Title: | Crystallization of the RNA guanylyltransferase of Chlorella virus PBCV-1 |
| Abstract: | RNA guanylyltransferase, or capping enzyme (E.C. 2.7.7.50) catalyzes the transfer of GMP from GTP to diphosphate-terminated RNA to form the cap structure GpppN. Chlorella virus cupping enzyme expressed in E. coli has been purified, treated with GTP and crystallized. X-ray diffraction data have been collected from these crystals as well as for a mercury derivative obtained by soaking the crystals in thimerosal. Selenomethionine RNA guanylytransferase was purified and crystallized in a similar fashion. The space group is C2221 and the cell parameters are a = 93.3, b = 214.9, c = 105.8 Å. Two Hg atoms and two subsets of Se atoms have been localized using difference Patterson and Fourier methods, suggesting that there are two molecules per asymmetric unit. |
| Keywords: | escherichia coli; chlorella virus; paramecium bursaria chlorella virus 1 |
| Journal Title: | Acta Crystallographica Section D: Biological Crystallography |
| Volume: | D53 |
| Issue: | 4 |
| ISSN: | 0907-4449 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1997-07-01 |
| Start Page: | 482 |
| End Page: | 484 |
| Language: | English |
| DOI: | 10.1107/s090744499700231x |
| PROVIDER: | scopus |
| PUBMED: | 15299921 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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