A global view of meiotic double-strand break end resection Journal Article
| Authors: | Mimitou, E. P.; Yamada, S.; Keeney, S. |
| Article Title: | A global view of meiotic double-strand break end resection |
| Abstract: | DNA double-strand breaks that initiate meiotic recombination are exonucleolytically processed. This 5'→3' resection is a central, conserved feature of recombination but remains poorly understood. To address this lack, we mapped resection endpoints genomewide at high resolution in Saccharomyces cerevisiae. Full-length resection requires Exo1 exonuclease and the DSB-responsive kinase Tel1, but not Sgs1 helicase. Tel1 also promotes efficient and timely resection initiation. Resection endpoints display pronounced heterogeneity between genomic loci that reflects a tendency for nucleosomes to block Exo1, yet Exo1 also appears to digest chromatin with high processivity and at rates similar to naked DNA in vitro. This paradox points to nucleosome destabilization or eviction as a defining feature of the meiotic resection landscape. © 2016 by the American Association for the Advancement of Science; all rights reserved. |
| Keywords: | saccharomyces cerevisiae |
| Journal Title: | Science |
| Volume: | 355 |
| Issue: | 6320 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 2017-01-06 |
| Start Page: | 40 |
| End Page: | 45 |
| Language: | English |
| DOI: | 10.1126/science.aak9704 |
| PROVIDER: | scopus |
| PMCID: | PMC5234563 |
| PUBMED: | 28059759 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 February 2017 -- Source: Scopus |
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