The genetic landscape of breast carcinomas with neuroendocrine differentiation Journal Article

Authors: Marchiò, C.; Geyer, F. C.; Ng, C. K. Y.; Piscuoglio, S.; De Filippo, M. R.; Cupo, M.; Schultheis, A. M.; Lim, R. S.; Burke, K. A.; Guerini-Rocco, E.; Papotti, M.; Norton, L.; Sapino, A.; Weigelt, B.; Reis-Filho, J. S.
Article Title: The genetic landscape of breast carcinomas with neuroendocrine differentiation
Abstract: Neuroendocrine breast carcinomas (NBCs) account for 2–5% of all invasive breast cancers, and are histologically similar to neuroendocrine tumours from other sites. They typically express oestrogen receptor (ER), and are HER2-negative and of luminal 'intrinsic' subtype. Here, we sought to define the mutational profile of NBCs, and to investigate whether NBCs and common forms of luminal (ER+/HER2−) breast carcinoma show distinct repertoires of somatic mutations. Eighteen ER+/HER2− NBCs, defined as harbouring >50% of tumour cells expressing chromogranin A and/or synaptophysin, and matched normal tissues were microdissected and subjected to massively parallel sequencing targeting all exons of 254 genes most frequently mutated in breast carcinomas and/or related to DNA repair. Their mutational repertoire was compared with that of ER+/HER2− breast carcinomas (n = 240), PAM50-defined luminal breast carcinomas (luminal A, n = 209; luminal B, n = 111) and invasive lobular carcinomas (n = 127) from The Cancer Genome Atlas. NBCs were found to harbour a median of 4.5 (range 1–11) somatic mutations, similar to that of luminal B breast carcinomas (median = 3, range 0–17) but significantly higher than that of luminal A breast carcinomas (median = 3, range 0–18, p = 0.02). The most frequently mutated genes were GATA3, FOXA1, TBX3, and ARID1A (3/18, 17%), and PIK3CA, AKT1, and CDH1 (2/18, 11%). NBCs less frequently harboured PIK3CA mutations than common forms of ER+/HER2−, luminal A and invasive lobular carcinomas (p < 0.05), and showed a significantly higher frequency of somatic mutations affecting ARID1A (17% versus 2%, p < 0.05) and the transcription factor-encoding genes FOXA1 (17% versus 2%, p = 0.01) and TBX3 (17% versus 3%, p < 0.05) than common-type ER+/HER2− breast carcinomas. No TP53 somatic mutations were detected in NBCs. As compared with common forms of luminal breast carcinomas, NBCs show a distinctive repertoire of somatic mutations featuring lower frequencies of TP53 and PIK3CA mutations, enrichment for FOXA1 and TBX3 mutations, and, akin to neuroendocrine tumours from other sites, ARID1A mutations. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Keywords: breast carcinoma; synaptophysin; chromogranin a; massively parallel sequencing; somatic mutations; neuroendocrine differentiation; copy number alterations
Journal Title: Journal of Pathology
Volume: 241
Issue: 3
ISSN: 0022-3417
Publisher: Wiley Blackwell  
Date Published: 2017-02-01
Start Page: 405
End Page: 419
Language: English
DOI: 10.1002/path.4837
PROVIDER: scopus
PUBMED: 27925203
PMCID: PMC5481202
Notes: Article -- Export Date: 2 February 2017 -- Source: Scopus
Citation Impact
MSK Authors
  1. Larry Norton
    671 Norton
  2. Britta Weigelt
    366 Weigelt
  3. Jorge Sergio Reis
    403 Reis
  4. Kiu Yan Charlotte Ng
    155 Ng
  5. Raymond Sear Lim
    56 Lim
  6. Caterina   Marchio
    26 Marchio
  7. Kathleen   Burke
    55 Burke