Fluorescein as a contrast agent for confocal intra-operative imaging of basal cell carcinomas: A preliminary ex vivo study Conference Paper


Authors: Sierra, H.; Qi, Q.; Jiang, A.; Taskar, N.; Rossi, A.; Rajadhyaksha, M.
Editors: Alfano, R. R.; Demos, S. G.
Title: Fluorescein as a contrast agent for confocal intra-operative imaging of basal cell carcinomas: A preliminary ex vivo study
Conference Title: Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis
Abstract: When used for intra-operative imaging of residual basal cell carcinomas (BCCs), reflectance confocal microscopy (RCM) is limited to detection of relatively large tumors. Small tumors remain hidden in the surrounding bright dermis. Fluorescence confocal microscopy (FCM) may improve the sensitivity for detecting small tumors. Fluorescein enhances cell cytoplasm contrast in fluorescence confocal images, but has had limited clinical impact on imaging BCCs in vivo because there is a lack of a well-defined protocol (concentration and application time) that can be effectively used for intraoperative imaging. We conducted an ex vivo study, using discarded tissue from Mohs surgery and a benchtop FCM with 488nm wavelength for excitation and 521nm detection for imaging Concentrations of 6, 0.6 and 0.6 mM with immersion times of 5, 15, 30, and 60 seconds were repeatedly tested (total of 76 specimens). The 0.6 mM and immersion time of 60 seconds showed that cellular cytoplasm can be labeled with controlled saturation and without leaving the yellow color on the surface of the tissue. Initial results show that, fluorescein may enhance cellular structures contrast relative to other normal dermal structures, improving the detection of small BCCs. This study provide an optimized set of parameters for subsequently testing of topical application in vivo for intraopertive imaging of BCCs. © 2016 SPIE.
Keywords: cytology; basal cell carcinoma; confocal microscopy; fluorescence; biopsy; tumors; diagnosis; reflectance confocal microscopies; contrast agent; tissue; cells; intra-operative imaging; cellular structure; dermal structure; fluorescence confocal microscopy; topical application
Journal Title Proceedings of SPIE
Volume: 9703
Conference Dates: 2016 Feb 15-17
Conference Location: San Francisco, CA
ISBN: 0277-786X
Publisher: SPIE  
Date Published: 2016-01-01
Start Page: 97030N
Language: English
DOI: 10.1117/12.2213719
PROVIDER: scopus
DOI/URL:
Notes: Conference Paper -- Conference code: 121891 -- Export Date: 3 January 2017 -- The Society of Photo-Optical Instrumentation Engineers (SPIE) -- 15 February 2016 through 17 February 2016 -- Source: Scopus
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  1. Anthony Rossi
    91 Rossi