Mass spectrometry of ribosomes and ribosomal subunits Journal Article
| Authors: | Benjamin, D. R.; Robinson, C. V.; Hendrick, J. P.; Hartl, F. U.; Dobson, C. M. |
| Article Title: | Mass spectrometry of ribosomes and ribosomal subunits |
| Abstract: | Nanoflow electrospray ionization has been used to introduce intact Escherichia coli ribosomes into the ion source of a mass spectrometer. Mass spectra of remarkable quality result from a partial, but selective, dissociation of the particles within the mass spectrometer. Peaks in the spectra have been assigned to individual ribosomal proteins and to noncovalent complexes of up to five component proteins. The pattern of dissociation correlates strongly with predicted features of ribosomal protein-protein and protein-RNA interactions. The spectra allow the dynamics and state of folding of specific proteins to be investigated in the context of the intact ribosome. This study demonstrates a potentially general strategy to probe interactions within complex biological assemblies. |
| Keywords: | nonhuman; protein conformation; mass spectrometry; protein assembly; protein protein interaction; molecular dynamics; protein binding; escherichia coli; protein biosynthesis; protein folding; structure analysis; molecular weight; protein determination; ribosomal proteins; ribosome protein; ribosomes; rna, bacterial; protein rna binding; ribosome; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 95 |
| Issue: | 13 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1998-06-23 |
| Start Page: | 7391 |
| End Page: | 7395 |
| Language: | English |
| DOI: | 10.1073/pnas.95.13.7391 |
| PUBMED: | 9636159 |
| PROVIDER: | scopus |
| PMCID: | PMC22627 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
