Isolation of neuronal precursors by sorting embryonic forebrain transfected with GFP regulated by the Tα1 tubulin promoter Journal Article
| Authors: | Wang, S.; Wu, H.; Jiang, J.; Delohery, T. M.; Isdell, F.; Goldman, S. A. |
| Article Title: | Isolation of neuronal precursors by sorting embryonic forebrain transfected with GFP regulated by the Tα1 tubulin promoter |
| Abstract: | Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an alternative strategy for the isolation and enrichment of precursor cells, by fluorescence-activated cell sorting of forebrain cells transfected with the gene for green fluorescent protein, driven by the neuronal Tα1 tubulin promoter. Using this approach, neural precursors and young neurons can be identified and selectively harvested from a variety of samples, including both avian and mammalian forebrains at different developmental stages. |
| Keywords: | controlled study; nonhuman; flow cytometry; biological markers; animal cell; phenotype; mammalia; animals; cells, cultured; embryo; green fluorescent protein; neurons; transfection; animalia; luminescent proteins; stem cell; gene expression regulation, developmental; aves; genetic transfection; rat; stem cells; green fluorescent proteins; rats; tubulin; forebrain; prosencephalon; nestin; nervous system development; nerve cell; cell separation; autoradiography; cell selection; chick embryo; chicken; promoter regions (genetics); stem cell biology; priority journal; article; gallus gallus; nneurogenesis |
| Journal Title: | Nature Biotechnology |
| Volume: | 16 |
| Issue: | 2 |
| ISSN: | 1087-0156 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1998-02-01 |
| Start Page: | 196 |
| End Page: | 201 |
| Language: | English |
| DOI: | 10.1038/nbt0298-196 |
| PUBMED: | 9487530 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
