| Abstract: |
We report the development of a reverse-transcriptase polymerase chain reaction assay that detects (in paraffin-embedded, formalin-fixed tissue) the SYT-SSX chimeric RNA transcript resulting from the t(X;18) of synovial sarcoma. The primers chosen detect both of the SSX1 and SSX2 partners, and the target sequence is small enough (87 base pairs) to be reliably detected in archival and variably processed consultation material. To demonstrate its usefulness, we applied it to 14 problematic cases, including spindle cell tumors of the thoracic region, of the neck, and of subcutaneous tissue. For instance, we show that, depending on the location, synovial sarcoma can mimic malignant solitary fibrous rumor, the spindle epithelial tumor with thymus-like differentiation, or skin adnexal tumors. Molecular detection of the SYT-SSX chimeric RNA should allow the reclassification of difficult cases in which the morphologic features overlap different entities or in which tumor nosology is still evolving. |
| Keywords: |
immunohistochemistry; adult; aged; proto-oncogene proteins; diagnostic accuracy; polymerase chain reaction; proteins; skin neoplasms; diagnosis, differential; neoplasm proteins; transcription, genetic; monoclonal antibody; thoracic neoplasms; chimera; recombinant fusion proteins; head and neck neoplasms; chromosome translocation; x chromosome; translocation, genetic; fibroma; base pairing; reliability; cancer classification; solitary fibrous tumor; synovial sarcoma; sarcoma, synovial; keratins; molecular probe techniques; repressor proteins; rna, neoplasm; epithelium tumor; tumor diagnosis; paraffin; paraffin embedding; rna analysis; chromosomal translocation; chromosomes, human, pair 18; humans; male; female; priority journal; article
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