A survey of the humoral immune response of cancer patients to a panel of human tumor antigens Journal Article
| Authors: | Stockert, E.; Jäger, E.; Chen, Y. T.; Scanlan, M. J.; Gout, I.; Karbach, J.; Arand, M.; Knuth, A.; Old, L. J. |
| Article Title: | A survey of the humoral immune response of cancer patients to a panel of human tumor antigens |
| Abstract: | Evidence is growing for both humoral and cellular immune recognition of human tumor antigens. Antibodies with specificity for antigens initially recognized by cytotoxic T lymphocytes (CTLs), e.g., MAGE and tyrosinase, have been detected in melanoma patient sera, and CTLs with specificity for NY- ESO-1, a cancer-testis (CT) antigen initially identified by autologous antibody, have recently been identified. To establish a screening system for the humoral response to autoimmunogenic tumor antigens, an enzyme-linked immunosorbent assay (ELISA) was developed using recombinant NY-ESO-1, MAGE- 1, MAGE-3, SSX2, Melan-A, and tyrosinase proteins. A survey of sera from 234 cancer patients showed antibodies to NY-ESO-1 in 19 patients, to MAGE-1 in 3, to MAGE-3 in 2, and to SSX2 in 1 patient. No reactivity to these antigens was found in sera from 70 normal individuals. The frequency of NY-ESO-1 antibody was 9.4% in melanoma patients and 12.5% in ovarian cancer patients. Comparison of tumor NY-ESO-1 phenotype and NY-ESO-1 antibody response in 62 stage IV melanoma patients showed that all patients with NY-ESO-1+ antibody had NY-ESO-1+ tumors, and no patients with NY-ESO-1- tumors had NY-ESO-1 antibody. As the proportion of melanomas expressing NY-ESO-1 is 20-40% and only patients with NY-ESO-1+ tumors have antibody, this would suggest that a high percentage of patients with NY-ESO-1+ tumors develop an antibody response to NY-ESO-1. |
| Keywords: | major clinical study; cancer diagnosis; neoplasms; ovarian neoplasms; proteins; melanoma; ovary cancer; breast cancer; lung neoplasms; neoplasm proteins; colonic neoplasms; membrane proteins; lung cancer; breast neoplasms; tumor antigen; enzyme linked immunosorbent assay; antigens, neoplasm; messenger rna; colon cancer; recombinant proteins; recombinant protein; cytotoxic t lymphocyte; antibody specificity; monophenol monooxygenase; enzyme-linked immunosorbent assay; humoral immunity; repressor proteins; autoantibodies; antibodies, neoplasm; complementary dna; antigen antibody complex; cancer immunology; humans; human; female; priority journal; article |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 187 |
| Issue: | 8 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1998-04-20 |
| Start Page: | 1349 |
| End Page: | 1354 |
| Language: | English |
| DOI: | 10.1084/jem.187.8.1349 |
| PUBMED: | 9547346 |
| PROVIDER: | scopus |
| PMCID: | PMC2212223 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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