Cloning and initial characterization of mouse meltrin β and analysis of the expression of four metalloproteasedisintegrins in bone cells Journal Article
| Authors: | Inoue, D.; Reid, M.; Lum, L.; Krätzschmar, J.; Weskamp, G.; Myung, Y. M.; Baron, R.; Blobel, C. P. |
| Article Title: | Cloning and initial characterization of mouse meltrin β and analysis of the expression of four metalloproteasedisintegrins in bone cells |
| Abstract: | Here we report the cloning and initial biochemical characterization of the mouse metalloprotease/disintegrin/cysteine-rich (MDC) protein meltrin β and the analysis of the mRNA expression of four MDC genes (meltrin α, meltrin β, mdc9, and mdc15) in bone cells, including osteoclasts and osteoblasts. Like most other MDC proteins, the predicted meltrin β protein consists of a signal sequence, prodomain, metalloprotease domain with a predicted catalytic site, disintegrin domain, cysteine-rich region, epidermal growth factor repeat, transmembrane domain, and cytoplasmic domain with putative signaling motifs, such as potential SH3 ligand domains. Northern blot analysis indicates that meltrin β is widely expressed, with the highest expression in bone, heart, and lung. RNase protection studies revealed expression of all four MDC genes analyzed here in osteoblasts, whereas only mdc9 and mdc15 mRNAs were detectable in osteoclast-like cells generated in vitro. Treatment of primary osteoblasts with 10 nM calcitriol increased meltrin β expression more than 3-fold, and both meltrin α and meltrin β expression is apparently regulated in a differentiation-associated manner in a mouse osteoblastic cell line, MC3T3E1. Collectively, these results suggest that meltrin α and meltrin β may play a role in osteoblast differentiation and/or function but are not likely to be involved in osteoclast fusion. |
| Keywords: | controlled study; protein expression; nonhuman; protein domain; protein analysis; animal cell; mouse; animals; mice; animal tissue; cell line; membrane proteins; cell differentiation; calcitriol; animalia; gene expression regulation; molecular cloning; cloning, molecular; amino acid sequence; molecular sequence data; rna, messenger; sequence alignment; signal peptide; osteoclast; osteoclasts; rodentia; cysteine; osteoblast; osteoblasts; metalloproteinase; bone cell; adam proteins; sequence analysis, dna; ribonuclease; metalloproteases; muscle proteins; disintegrin; disintegrins; metalloendopeptidases; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 273 |
| Issue: | 7 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1998-02-13 |
| Start Page: | 4180 |
| End Page: | 4187 |
| Language: | English |
| DOI: | 10.1074/jbc.273.7.4180 |
| PUBMED: | 9461614 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work