Role of the core DNA polymerase III subunits at the replication fork: α is the only subunit required for processive replication Journal Article

  


Authors: Marians, K. J.; Hiasa, H.; Kim, D. R.; McHenry, C. S.
Article Title: Role of the core DNA polymerase III subunits at the replication fork: α is the only subunit required for processive replication
Abstract: The DNA polymerase III holoenzyme is composed of 10 subunits. The core of the polymerase contains the catalytic polymerase subunit, α, the proofreading 3' → 5' exonuclease, ε, and a subunit of unknown function, Θ. The availability of the holoenzyme subunits in purified form has allowed us to investigate their roles at the replication fork. We show here that of the three subunits in the core polymerase, only α is required to form processive replication forks that move at high rates and that exhibit coupled leading- and lagging-strand synthesis in vitro. Taken together with previous data this suggests that the primary determinant of replication fork processivity is the interaction between another holoenzyme subunit, τ, and the replication fork helicase, DnaB.
Keywords: dna-binding proteins; nonhuman; dna replication; dna synthesis; enzyme activity; bacterial proteins; dna; dimerization; molecular interaction; catalysis; nucleic acid conformation; enzyme subunit; dna, bacterial; dna helicases; base mispairing; dna template; dna directed dna polymerase gamma; templates, genetic; dna content; dnab helicases; dna polymerase iii; priority journal; article
Journal Title: Journal of Biological Chemistry
Volume: 273
Issue: 4
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 1998-01-23
Start Page: 2452
End Page: 2457
Language: English
DOI: 10.1074/jbc.273.4.2452
PUBMED: 9442096
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031892179&doi=10.1074%2fjbc.273.4.2452&partnerID=40&md5=fe4502d824ad6c7f347304c7c0a1850e
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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MSK Authors
  1. Kenneth Marians
    138 Marians
  2. Hiroshi Hiasa
    21 Hiasa
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