Characterization of a mouse monoclonal IgG3 antibody to the tumor-associated globo H structure produced by immunization with a synthetic glycoconjugate Journal Article

  

Authors:	Kudryashov, V.; Ragupathi, G.; Kim, I. J.; Breimer, M. E.; Danishefsky, S. J.; Livingston, P. O.; Lloyd, K. O.
Article Title:	Characterization of a mouse monoclonal IgG3 antibody to the tumor-associated globo H structure produced by immunization with a synthetic glycoconjugate
Abstract:	Globo H (Fucα1 → 2Galβ1 → 3GalNAcβ1 → 3Galα1 → 4Galβ1 → 4Glc) is a carbohydrate structure that shows enhanced expression in many human carcinomas. From mice immunized with a globo H-KLH (keyhole limpet hemocyanin) synthetic conjugate an IgG3 monoclonal antibody (mAb VK-9) was derived that recognizes the globo H structure. Serological analysis showed that the minimal structure recognized by this mAb was the tetrasaccharide sequence Fucα1 → 2Galβ1 → 3GalNAcβ1 → 3Gal. An isomeric structure with an internal αGalNAc linkage was also recognized but less efficiently. mAb VK-9 did not react with many related structures, such as galactosylgloboside, globoside, H type 1, H type 2 blood group structures or fucosyl-gangliotetraosyl ceramide, but did react weakly with globo A ceramide. Not only did mAb VK-9 react with carbohydrate-protein conjugates but it could also recognize globo H-ceramide and human tumor cells expressing globo H. These results suggest that globo H-KLH could be explored as a vaccine in the treatment of carcinoma patients.
Keywords:	controlled study; human cell; nonhuman; animal cell; mouse; animals; mice; animal experiment; tumor antigen; monoclonal antibody; antibodies, monoclonal; molecular sequence data; immunoglobulin g; cancer vaccines; antigen recognition; tumor cell line; antibody specificity; immunoglobulin g3; serology; carbohydrate analysis; antigens, tumor-associated, carbohydrate; carbohydrate sequence; keyhole limpet hemocyanin; ceramide; hemocyanin; vaccines, conjugate; immunization; oligosaccharide; glycolipid; glycoconjugate; conjugate; glycoconjugates; globo h; humans; human; female; priority journal; article; synthetic oligosaccharides; globoside
Journal Title:	Glycoconjugate Journal
Volume:	15
Issue:	3
ISSN:	0282-0080
Publisher:	Springer New York LLC
Date Published:	1998-03-01
Start Page:	243
End Page:	249
Language:	English
DOI:	10.1023/a:1006992911709
PUBMED:	9579801
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031937646&doi=10.1023%2fA%3a1006992911709&partnerID=40&md5=c3e8e0ec7a0d25a29048d8daa42d42c0
Notes:	Article -- Export Date: 12 December 2016 -- Source: Scopus

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