Double-strand break repair by interchromosomal recombination: Suppression of chromosomal translocations Journal Article

   


Authors: Richardson, C.; Moynahan, M. E.; Jasin, M.
Article Title: Double-strand break repair by interchromosomal recombination: Suppression of chromosomal translocations
Abstract: To directly determine whether recombinational repair of double-strand breaks (DSBs) can occur between heterologous chromosomes and lead to chromosomal rearrangements in mammalian cells, we employed an ES cell system to analyze recombination between repeats on heterologous chromosomes. We found that recombination is induced at least 1000-fold following the introduction of a DSB in one repeat. Most (98%) recombinants repaired the DSB by gene conversion in which a small amount of sequence information was transferred from the unbroken chromosome onto the broken chromosome. The remaining recombinants transferred a larger amount of information, but still no chromosomal aberrations were apparent. Thus, mammalian cells are capable of searching genome-wide for sequences that are suitable for DSB repair. The lack of crossover events that would have led to translocations supports a model in which recombination is coupled to replication.
Keywords: controlled study; mutation; proto-oncogene proteins; nonhuman; dna replication; animal cell; mouse; mammalia; animals; mice; dna damage; in situ hybridization, fluorescence; dna repair; cell line; alleles; transfection; animalia; stem cell; dna strand breakage; gene conversion; fluorescence in situ hybridization; double stranded dna; genetic recombination; protein-serine-threonine kinases; gene duplication; chromosome rearrangement; chromosome translocation; translocation, genetic; models, genetic; genes, reporter; sequence homology, nucleic acid; chromosomes; es cells; embryo cell; mammal cell; recombination; crossing over, genetic; translocation; proto-oncogene proteins c-pim-1; deoxyribonucleases, type ii site-specific; dna double-strand break; blotting, southern; priority journal; article
Journal Title: Genes and Development
Volume: 12
Issue: 24
ISSN: 0890-9369
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 1998-12-15
Start Page: 3831
End Page: 3842
Language: English
PUBMED: 9869637
PROVIDER: scopus
PMCID: PMC317271
DOI: 10.1101/gad.12.24.3831
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032535036&partnerID=40&md5=9d5b189d14cf883e0f28af9e6a782f95
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Mary Ellen Moynahan
    105 Moynahan
  2. Maria Jasin
    249 Jasin
  3. Christine Richardson
    13 Richardson
Related MSK Work