The structure of supercoiled intermediates in DNA replication Journal Article
| Authors: | Peter, B. J.; Ullsperger, C.; Hiasa, H.; Marians, K. J.; Cozzarelli, N. R. |
| Article Title: | The structure of supercoiled intermediates in DNA replication |
| Abstract: | We studied the structure of replication intermediates accumulated by Tus-induced arrest of plasmid DNA replication at termination sites. For intermediates generated both in vitro with purified components and in vivo, superhelical stress is distributed throughout the entire partially replicated molecule; daughter DNA segments are wound around each other, and the unreplicated region is supercoiled. Thus, unlinking of parental DNA strands by topoisomerases can be carried out both behind and in front of the replication fork. We explain why previous studies with prokaryotic and eukaryotic replication intermediates discerned only supercoiling in the unreplicated portion. |
| Keywords: | nonhuman; dna replication; protein domain; microscopy, electron; escherichia coli; plasmids; nucleic acid conformation; eukaryote; dna topoisomerase; plasmid dna; dna conformation; dna supercoiling; dna, superhelical; prokaryote; dna strand; isomerism; priority journal; article; electrophoresis, agar gel |
| Journal Title: | Cell |
| Volume: | 94 |
| Issue: | 6 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1998-09-18 |
| Start Page: | 819 |
| End Page: | 827 |
| Language: | English |
| DOI: | 10.1016/s0092-8674(00)81740-7 |
| PUBMED: | 9753328 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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