Antisense mapping of opioid receptor clones: Effects upon 2-deoxy-D-glucose-induced hyperphagia Journal Article

  


Authors: Burdick, K.; Yu, W. Z.; Ragnauth, A.; Moroz, M.; Pan, Y. X.; Rossi, G. C.; Pasternak, G. W.; Bodnar, R. J.
Article Title: Antisense mapping of opioid receptor clones: Effects upon 2-deoxy-D-glucose-induced hyperphagia
Abstract: Antisense oligodeoxynucleotides (AS ODNs) directed against exons 1 and 2 of the MOR-1 clone significantly and markedly reduced (81-93%) hyperphagia induced by the anti-metabolic glucose analogue, 2-deoxy-D-glucose (2DG) across a 4 h time course. AS ODNs directed against exons 3 or 4 of the MOR-1 clone had a more limited (1-2 h) duration of action upon 2DG-induced hyperphagia. 2DG-induced hyperphagia was significantly reduced by AS ODNs directed against exon 2 (44-51%), but not exons 1 or 3 of the KOR-1 clone across a 4 h time course. Whereas an AS ODN probe directed against the KOR3/ORL-1 clone produced small (36%), but significant reductions in 2DG- induced hyperphagia, an AS ODN probe directed against the DOR-1 clone was ineffective. These data provide further converging evidence for the roles of primarily mu, but also kappa, and kappa3 opioid receptors in mediating the hyperphagic effects of glucoprivation.
Keywords: controlled study; exons; nonhuman; animals; animal experiment; weight reduction; feeding; food intake; cloning, molecular; rat; rats; rats, sprague-dawley; mu opiate receptor; opiate receptor; oligonucleotides, antisense; delta opiate receptor; kappa opiate receptor; antisense oligodeoxynucleotide; deoxyglucose; receptors, opioid; intracerebroventricular drug administration; male; priority journal; article; hyperphagia; glucoprivation; 2-deoxy-d-glucose; dor-1 clone; kor-1 clone; kor-3/orl-1 clone; mor-1 clone
Journal Title: Brain Research
Volume: 794
Issue: 2
ISSN: 0006-8993
Publisher: Elsevier Science, Inc.  
Date Published: 1998-06-01
Start Page: 359
End Page: 363
Language: English
DOI: 10.1016/s0006-8993(98)00331-x
PUBMED: 9622675
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032104016&doi=10.1016%2fS0006-8993%2898%2900331-X&partnerID=40&md5=ca41c78def0ccbb9f83d5ed22cbda683
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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MSK Authors
  1. Yingxian Pan
    133 Pan
  2. Grace Rossi
    61 Rossi
  3. Gavril W Pasternak
    415 Pasternak
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