A proportion of primary squamous cell carcinomas of the parotid gland harbour high-risk human papillomavirus Journal Article
| Authors: | Xu, B.; Wang, L.; Borsu, L.; Ghossein, R.; Katabi, N.; Ganly, I.; Dogan, S. |
| Article Title: | A proportion of primary squamous cell carcinomas of the parotid gland harbour high-risk human papillomavirus |
| Abstract: | Aims: In the current study, we aimed to examine primary parotid squamous cell carcinoma (ParSCC) for the presence of high-risk human papillomavirus (HR-HPV) and associated molecular alterations. Methods and results: Eight cases of ParSCC were retrieved after a detailed clinicopathological review to exclude the possibility of metastasis and/or extension from another primary site. HR-HPV status was determined on the basis of immunohistochemistry (IHC) for p16 expression and chromogenic in-situ hybridization (CISH) for HR-HPV. All cases were genotyped with a multiplexed mass spectrometry assay interrogating 91 hotspot mutations in eight cancer-related genes (EGFR, KRAS, NRAS, BRAF, PIK3CA, AKT1, MEK1 and ERBB2), and studied by fluorescence in-situ hybridization for PTEN copy number alteration. Three of eight cases (37.5%) were positive for the presence of HR-HPV by CISH and p16 IHC. One of three (33%) HR-HPV-positive cases harboured a PTEN hemizygous deletion, and one (33%) HR-HPV-positive case harboured a PIK3CA E545K somatic mutation. No alteration of the PTEN–PI3K pathway was detected in HR-HPV-negative tumours. Over a median follow-up period of 66.2 months, only the patient with the HR-HPV-positive PIK3CA-mutated tumour died of his disease, the remaining seven patients being disease-free. Conclusions: Given the established aetiological role of HR-HPV in other head and neck squamous cell carcinomas, it is likely that HR-HPV represents an oncogenic driver in the pathogenesis of more than one-third of ParSCCs. The presence of HR-HPV in ParSCC may be coupled with alterations in the PTEN–PI3K pathway. Further studies on HR-HPV and the molecular characterization of a larger number of ParSCCs are needed to determine the clinical significance of these findings. © 2016 John Wiley & Sons Ltd |
| Keywords: | squamous cell carcinoma; parotid gland; p16; in-situ hybridization; human papillomavirus |
| Journal Title: | Histopathology |
| Volume: | 69 |
| Issue: | 6 |
| ISSN: | 0309-0167 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2016-12-01 |
| Start Page: | 921 |
| End Page: | 929 |
| Language: | English |
| DOI: | 10.1111/his.13027 |
| PROVIDER: | scopus |
| PMCID: | PMC5115934 |
| PUBMED: | 27374168 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 December 2016 -- Source: Scopus |
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