Gel electrophoresis assays for analyzing DNA double-strand breaks in Saccharomyces cerevisiae at various spatial resolutions Journal Article

   

Authors:	Murakami, H.; Borde, V.; Nicolas, A.; Keeney, S.
Article Title:	Gel electrophoresis assays for analyzing DNA double-strand breaks in Saccharomyces cerevisiae at various spatial resolutions
Abstract:	Meiotic recombination is triggered by programmed DNA double-strand breaks (DSBs), which are catalyzed by Spo11 protein in a type II topoisomerase-like manner. Meiotic DSBs can be detected directly using physical assays (gel electrophoresis, Southern blotting, and indirect end-labeling) applied to samples of genomic DNA from sporulating cultures of budding and fission yeast. Such assays are extremely useful for quantifying and characterizing many aspects of the initiation of meiotic recombination, including the timing of DSB formation relative to other events, the distribution of DSBs across the genome, and the influence on DSB formation of mutations in recombination factors and other gene products. By varying the type of gel electrophoresis and other parameters, the spatial resolution of DSB analysis can range from single nucleotides up to whole yeast chromosomes.
Keywords:	genetics; review; molecular genetics; methodology; chromosome; biological model; models, biological; algorithms; molecular sequence data; algorithm; saccharomyces cerevisiae; nucleotide sequence; dna breaks, double-stranded; double stranded dna break; base sequence; dna mutational analysis; genetic techniques; genetic procedures; chromosome map; pulsed field gel electrophoresis; chromosome mapping; chromosomes, fungal; electrophoresis, gel, pulsed-field
Journal Title:	Methods in Molecular Biology
Volume:	557
ISSN:	1064-3745
Publisher:	Humana Press Inc
Date Published:	2009-01-01
Start Page:	117
End Page:	142
Language:	English
DOI:	10.1007/978-1-59745-527-5_9
PUBMED:	19799180
PROVIDER:	scopus
PMCID:	PMC3157973
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-70350786471&partnerID=40&md5=a2ea220b6853e1b0d3568336e3135f01
Notes:	Chapter 9 in "Meiosis: Volume 1, Molecular and Genetic Methods" (ISBN: 978-1-934115-66-4) --- Export Date: 30 November 2010 - Source: Scopus

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